Contact an Account ManagerDirect liquid chromatographic separation of enantiomers on immobilized protein stationary phases. IV. Molecular interaction forces and retention behaviour in chromatography on bovine serum albumin as
Summary
Type: HPLC
Application #: 105490
Catalog#: 720046.40 - MN HPLC Column, Resolvosil BSA-7, 300A, 7um, 4x150mm
Detection: Fig. 3: UV, 225nm, 0.1 AUFS
Fig. 4: UV, left 225nm, right 269nm, 0.05 AUFS
Conditions: Fig. 3: eluent: 50 mM phosphate buffer pH 6.0 + 2% 1-propanol, flow rate 1.0ml/min
Fig. 4: eluent: 40mM phosphate buffer pH 6.6 + 3% 1-propanol, flow rate 1.0ml/min
Authors: Allenmark S
Source: J Chromatogr 316 (1984) 617 - 624
Details
Legend: Fig. 3. Chromatogram showing the large difference in a-value between N-formyl-
(Va) and N-acetyl-D,L-tryptophan (Vb), samples 0.1mM each, 20µl injected.
Fig. 4. Chromatogram showing the effect of